Introduction to Real-Time PCR
The HSA Islamabad is a premier institution of the Ministry of Health in Pakistan recognized by PMDC and HEC
Course Instructors
Mr. Sheraz Younas
With 20+ years in lab diagnostics, SOPs development, and ISO 15189-12 Technical Assessor for PNAC. Certified Biosafety Officer & Master Trainer by International Federation of Biosafety Associations (IFBA), American Society for Microbiology (ASM) and Pakistan Biological Safety Association (PBSA). His national and international experience and certifications highlight his commitment to lab quality and safety.
Course Outline
- Overview of PCR
- Principles of real-time PCR: What is qPCR?
- Overview of qPCR instruments and their components
- Selection of reagents: enzymes, buffers, dyes, and probes
- Sample preparation and nucleic acid extraction
- Understanding qPCR data output (Ct values, amplification plots)
- Applications of qPCR in research and diagnostics
- Identifying and addressing common data issues
- Template quality and quantity considerations
- Strategies for optimization and improving reproducibility
- Inhibitors and their effects on qPCR
- Primer efficiency and specificity
Eligibility Criteria
All Healthcare Professionals working in labs including Doctors & Allied Health Sciences professional
Contact Details
learning@nhs-neqas.com
0300-5012268
